ASSESSMENT OF TRIMETHOPRIM -LOAD SOLD LIPID NANOPARTICLES ACTIVITY AGAINST STAPH AUROUS ISOLATED FROM URINARY TRACT INFECTIONS IN WOMEN

Abstract

Objective: to Isolate Staph aurous from urinary tract infection in women and study in the vitro antibiotic of the standard Trimethoprim and Trimethoprim loaded solid lipid nanoparticles formulation against Staph aurous isolated. Method: A total of 123 urine samples from women patients with urinary tract infections were gathered, from different hospitals around waist province from 15 June for 15 September, 2023. Morphological characteristics and biochemical tests based on Api20, Vitek 2 system, and Bogy's Manual of Systematic Bacteriology were used to identify the samples Result: The results were 60 (48.7%) isolates of Staph aurous obtained from 123 from women with urinary tract infections (UTI), distributed as symptomatic 71(57.7%) and asymptomatic 52 (42.3%). In-vitro Antibacterial Activity the activity of TMP-SLNs formulation and standard TMP against UTI isolates of Staph aurous was sensitive to the tested formulations. The TMP-SLNs formulation shown considerably greater (p<0.05) antibacterial activity in terms of zone of inhibition against the bacteria. When compared to typical TMP values, the MIC and MBC values of the TMP-SLNs formulation against Staph. aurous were almost sixteen times higher. This indicates that TMP's antibacterial action is enhanced when it is loaded into SLNs. Conclusions: the result of study highlight that Trimethoprim -load sold lipid nanoparticles it might be an excellent option for the sustained delivery of Antibacterial against Staph aurous isolated.